Journal of Bioscience and Bioengineering Vol. 100, No. 4 (October 2005) Cover

Finding of a new family of NAD(P)H-dependent oxidoreductases with no Rossmann-fold.

The Overall structure of Δ1-piperideine-2-carboxylate (Pip2C)/Δ1-pyrroline-2-carboxylate (Pyr2C) reductase from Pseudomonas syringae (left) is distinct from that of a typical Rossmann-fold enzyme, malate dehydrogenase from Escherichia coli (right). α-Helices and α-sheets are shown in red and blue, respectively. NADP+ (left) and NAD+ (right) molecules are in green.

The new NAD(P)H-dependent oxidoreductase family proteins which have no Rossmann-fold were classified into eight clades. Pip2C/Pyr2C reductase belongs to the DpkA clade in the new family. This classification would be useful for reliable functional annotation of the new family of NAD(P)H-dependent oxidoreductases.

Related article: Muramatsu, H., Mihara, H., Goto, M., Miyahara, I., Hirotsu, K., Kurihara, T., and Esaki, N., "A new family of NAD(P)H-dependent oxidoreductases distinct from conventional Rossmann-fold proteins", J. Biosci. Bioeng., vol. 99, 541-547 (2005).