Journal of Bioscience and Bioengineering Vol. 106 No. 3  (September 2008)  Cover

Enrichment of carotenoids in flaxseed.

Left: an untransformed flax plant (WARD cultivar). The transgenic flax plants were the same to untransformed control plants in their appearance except for seed color.

Right top: section of an untransformed flaxseed.

Right below three: sections of transgenic flaxseeds. These transgenic plants were generated by introduction of the phytoene synthase gene (crtB) derived from soil bacterium Pantoea ananatis (formerly called Erwinia uredovora 20D3). 

The inner color of the transgenic flaxseeds was altered into orange due to the accumulation of β-carotene and α-carotene. Total carotenoid amounts in these seeds were 65.4-156.3 μg/g fresh weight, which corresponded to 7.8- to 18.6-fold increase, compared with those of untransformed controls. 

Related article: Fujisawa, M., Watanabe, M., Choi, S.-K., Teramoto, M., Ohyama, K., and Misawa, N., "Enrichment of carotenoids in flaxseed (Linum usitatissimum) by metabolic engineering with introduction of bacterial phytoene synthase gene crtB" J. Biosci. Bioeng., vol. 105, 636-641 (2008).